Tissue Culture Techniques for Rapid Propagation of Selected Cassava

This study was conducted in the Plant Tissue Culture Laboratory of the Agricultural Research Corporation (ARC) from 2005 to 2008 to develop in vitro propagation techniques for cassava genotypes. The effects of different nutrient media formulations) Gamborg et al., 1968 called B5; Murashige and Skoog, 1962 called MS and Schenk and Hildebrandt, 1968 called SH) on micropropagation were tested. Murashige and Skoog was the best medium for propagation of cassava and the cassava genotype Abili had the best shoot morphogenesis. Different ratios of wheat flour and agar (70g flour, 70g/2g, 60g/3g, 40g/4g, and 8g/l agar) as nutrient media supporting gelling agent were tested on four cassava genotypes.