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Parallel Single Cell Electrophoresis in a Microchip Platform
- Editore:
LAP Lambert Academic Publishing
- EAN:
9783838304489
- ISBN:
3838304489
- Pagine:
- 140
- Formato:
- Paperback
- Lingua:
- Tedesco
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Descrizione Parallel Single Cell Electrophoresis in a Microchip Platform
Single cell analysis has typically been difficult and challenging to perform in such a way that meaningful statistical information can be extracted. This thesis documents the development of a microchip or lab-on-a-chip platform to perform single cell electrophoresis in parallel separation channels. It describes the use of optical tweezers to select a cell and transport it to a desired separation channel. It also describes the use of electromechanical shearing to lyse a cell. These two techniques are combined with laser-micromachined separation channels to perform simple electrohoresis on the fluorescently labeled cellular contents.
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