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Oligonucleotide Synthesis
Methods And Applications
- Editore:
Humana
- Collana:
- Methods In Molecular Biology
- EAN:
9781617374418
- ISBN:
1617374415
- Pagine:
- 436
- Formato:
- Paperback
- Lingua:
- Inglese
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Descrizione Oligonucleotide Synthesis
A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
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