Molecular Characterisation of the Ribgrass MosaicVirus - Impatiens
- Editore:
VDM Verlag Dr. Müller e.K.
- EAN:
9783639090284
- ISBN:
3639090284
- Pagine:
- 112
- Formato:
- Paperback
- Lingua:
- Tedesco
Descrizione Molecular Characterisation of the Ribgrass MosaicVirus - Impatiens
Regarding a strategy of expressing foreign genes inplants, two diverged RMV-based expression vectorscontaining a green fluorescent protein were clonedinto the binary vector pPZP200 for subsequentagroinfiltration work. An infectious full-lengthclone of RMV and three RMV-based mutants alreadyexisted. They vary depending on which of the twoputative ORFs is inactivated. An additional mutationsite of one of these mutation constructs wasdiscovered and reverse mutated. After the inoculationof the constructs on N. benthamiana and N. tabacum,the analysis revealed distinct effects in virusaccumulation and symptom characteristics of the threemutation constructs. These results differ from theones of the wild type clone. Accordingly, all threemutants show both lower virus accumulation levels andlesser symptom characteristics. Further, the resultsfrom N. benthamiana indicate an ability to reversemutate the -4K virus. In addition to the descriptionand the analysis of these results, the potential roleof the two putative ORFs will be discussed and anoutlook on further investigations will be given.
