In-cell approaches in electron paramagnetic resonance spectroscopy di Mykhailo Azarkh edito da Südwestdeutscher Verlag für Hochschulschriften AG  Co. KG
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In-cell approaches in electron paramagnetic resonance spectroscopy

In-cell approaches in electron paramagnetic resonance spectroscopy to study conformations of DNA G-quadruplexes

EAN:

9783838134406

ISBN:

3838134400

Pagine:
152
Formato:
Paperback
Lingua:
Tedesco
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Descrizione In-cell approaches in electron paramagnetic resonance spectroscopy

Double electron electron resonance (DEER) is an EPR technique which allows for distance measurements on the nanometer scale. Here, EPR-based distance measurements were extended to in-cell applications. From evaluation of nitroxides for experiments in intracellular environment of Xenopus laevis oocytes and through proof-of-principle experiments optimal parameters for performing in-cell DEER were found. On the example of G-quadruplexes formed by oligomers of human telomeric DNA, DEER was shown to be applicable for structural identification of biological objects under their native conditions.

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