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In-cell approaches in electron paramagnetic resonance spectroscopy
In-cell approaches in electron paramagnetic resonance spectroscopy to study conformations of DNA G-quadruplexes
- EAN:
9783838134406
- ISBN:
3838134400
- Pagine:
- 152
- Formato:
- Paperback
- Lingua:
- Tedesco
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Descrizione In-cell approaches in electron paramagnetic resonance spectroscopy
Double electron electron resonance (DEER) is an EPR technique which allows for distance measurements on the nanometer scale. Here, EPR-based distance measurements were extended to in-cell applications. From evaluation of nitroxides for experiments in intracellular environment of Xenopus laevis oocytes and through proof-of-principle experiments optimal parameters for performing in-cell DEER were found. On the example of G-quadruplexes formed by oligomers of human telomeric DNA, DEER was shown to be applicable for structural identification of biological objects under their native conditions.
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