Understanding NMR Spectroscopy

16Understanding NMR SpectroscopyJames Keeler Department of Chemistry, University of Cambridge, UKThis text discusses the high-resolution NMR of liquid samples and concentrates exclusively on spin-half nuclei (mainly 1H and 13C). It is aimed at people who are familiar with the use of routine NMR for structure determination and who wish to deepen their understanding of just exactly how NMR experiments work. It demonstrates that in NMR it is possible, quite literally on the back of an envelope, to make exact predictions of the outcome of quite sophisticated experiments. The experiments chosen are likely to be encountered in the routine NMR of small to medium-sized molecules, but are also applicable to the study of large biomolecules, such as proteins and nucleic acids.The book starts off at a gentle pace, working through some more-or-less familiar ideas, and then elaborating these as the book progresses. Each chapter ends with exercises which are designed to assist in the understanding16 of the ideas presented and to grasp the underlying ideas.041. What this book is about and who should read it.2. Setting the scene.3. Energy levels and NMR spectra.4. The vector model.5. Fourier transformation and data processing.6. The quantum mechanics of one spin.7. Product operators.8. Two-dimensional NMR.9. Relaxation and the NOE.10. Advanced topics in tow-dimensional NMR.11. Coherence selection: phase cycling and field gradient pulses.12. How the spectrometer works.Appendix: Some mathematical topics.Index.